ABSTRACT
Abstract The main aim of the study is to quantify the cytotoxic property of the Fucoidan extracted from the Turbinaria conoides using the MTT assay with the standard fucose. Fucoidan was extracted using the soaked water method and it was determined using the HPLC procedure the obtained Test sample Fucoidan extracted from the Turbinaria conoides and standard fucose was subjected to the cytotoxicity assay against the MCF7 Human breast cancer cell line, A549 lung cancer cell line, and L929 normal mouse fibroblast cell line. From the results it was found that the Test sample showed good IC50 value for MCF7 cell line then A549 with an increasing concentration 24 hours incubation at 37°C The IC50 for MCF7 was 115.21 µg/ml and A549 396.46µg/ml and the Fucoidan extract was checked for its cytotoxicity against the normal mouse fibroblast cell line L929, Fucoidan was found non-lethal to the L929 mouse fibroblast normal cell line. Standard fucose also gave a significant result towards MCF7 and against the L929. This indicates that the Fucoidan extracted from Tubinaria conoides shows better anticancer potential in it. Hence its application can be further extended in the pharmacological fields.
Subject(s)
In Vitro Techniques/instrumentation , Cytotoxins/adverse effects , MCF-7 Cells , A549 Cells , Breast Neoplasms/pathology , Cell Line , Chromatography, High Pressure Liquid/methods , Inhibitory Concentration 50 , Fibroblasts/classification , Fucose/analogs & derivatives , Lung Neoplasms/pathologyABSTRACT
Objective: To isolate, culture and characterize fibroblasts from the buccal mucosa of areca nut chewers with and without oral submucous fibrosis (OSF). Materials and Methods: Primary fibroblast cultures were established by the collagenase disaggregation technique and the phenotypic and growth characteristics were studied. Results: Cells cultured from OSF showed a statistically significant increase in both the post-mitotic fibroblast subpopulation and the population doubling time when compared with controls. Conclusion: There was a significant increase in the pro-fibrotic, post-mitotic subpopulation of fibroblasts in areca nut chewers with OSF.
Subject(s)
Adolescent , Adult , Animals , Areca , Cell Count , Cell Culture Techniques , Cell Line , Cell Proliferation , Cell Separation , Cell Shape , Collagenases , Female , Fibroblasts/classification , Fibroblasts/pathology , Humans , Male , Middle Aged , Mitosis/physiology , Mouth Mucosa/pathology , Oral Submucous Fibrosis/pathology , Phenotype , Rats , Time Factors , Young AdultABSTRACT
Se reporta un caso de lactante mayor femenino de 2 meses de edad, portador de lesiones cutáneas hipocrómicas, congénitas, con alteración del desarrollo musculoesquelético y alteraciones neurológicas leves. El hallazgo histopatológico de las lesiones cutáneas fue compatible con Hipomelanosis de Ito. El cariotipo de fibroblastos de piel y de linfocitos de sangre periférica se reportó como 46, XX normal. Se revisa en la literatura los reportes de alteraciones extracutáneas asociadas y criterios diagnósticos